| Name | Support | Language | Availability | Edition date | Price | ||
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PDF |
English |
Active |
9/23/2020 |
€163.00 |
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Details
This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay utilizing stably transfected human cells. This reporter gene assay is based on the activation of the human estrogen receptor alpha. This method is applicable to: — fresh water — waste water — aqueous extracts and leachates — eluates of sediments (fresh water) — pore water — aqueous solutions of single substances or of chemical mixtures — drinking water — the limit of quantification (LOQ) of this method for the direct analysis of water samples is between 0,3 ng/l and 1 ng/l 17ß-estradiol equivalents (EEQ) based on the results of the international interlaboratory trial (see Annex F). The upper working range was evaluated [based on the results of the international interlaboratory trial (see Table F.3)] up to a level of 75 ng EEQ/l. Samples showing estrogenic potencies above this threshold have to be diluted for a valid quantification. Extraction and pre concentration of water samples can prove necessary if their estrogenic potential is below the given LOQ.
Directives : Not yet available in DB
Additional Info
| Author | Bureau de Normalisation Belge (NBN) |
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| Published by | NBN |
| Document type | Standard |
| ICS | 13.060.70 : Examination of biological properties of water
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| Document history | No |
| Keyword | ISO 19040-3, ISO 19040-3:2020 |
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